Crandall Lab

Sequencing Reaction

	1/8 rxn	1/16 rxn	1/32 rxn
Big Dye	1.0ul	0.5ul	0.25ul
5x buffer	1.5ul	2.1ul	2.1ul
Primer	1.0ul	1.0ul	0.5ul
DNA	2.0ul	2.0ul	1.5ul
H2O	4.5ul	6.4ul	7.65ul
Total	10.0ul	10.0ul	12.0ul

Use either 1/16 th or 1/32th reaction sizes

Use 7-10pmoles primer per reaction

Use 30-50ng PCR product