Crandall Lab

1.    Bring total PCR volume up to 100ul with dH2O.

2.    Transfer all 100ul of diluted PCR reaction to a PCR 96 well clean up plate

3.    Put plate on vacuum manifold for 5 minutes until wells are empty, making sure pressure gauge is at -22 in Hg

• Wells will appear shiny, so they will look slightly wet all the time.

4.    Blot bottom of plate on paper towel to remove excess water

5.    Add 100ul of water to plate and re-vacuum plate

6.    Resuspend DNA with 30ul water

7.    Place plate on shaker / mixer for 10 minutes (or longer depending on concentration desired)

8.    Pipette product out of wells and transfer to clean labeled tube or plate.