Crandall Lab

PCR Reactions

 

1x28 Hotmaster PRC Reaction			1x25 PCR Reaction
10x PCR buffer	2.8ul		10xPCR Buffer	2.5ul
dNTPs	4.0ul		MgCl 25uM	4-5ul
Forward Primer	2.0ul		dNTPs	4.0ul
Reverse Primer	2.0ul		Forward Primer	2.5ul
Taq	0.15ul		Reverse Primer	2.5ul
DNA	~3ul		Taq	0.15ul
Water	14.05ul		DNA	0.5-2ul
Total Volume	28.0ul		Water	difference
			Total Volume	25

Check of PCR Reactions