Crandall Lab

1.    Make 1.0% agarose gel

• 0.7 grams Agarose, 70mL 1x TAE buffer.  Mix and microwave for 2 min until agarose is in solution.  Let cool to warm to touch and add Sybr safe and pour into casting tray.  Push any bubbles to the sides of the tray.  Insert combs.  Allow 20 minutes for gel to set up.

2.    Pipette small amount of bromophenol blue dye onto a piece of parafilm (~ 1ul)

3.    Add 3ul of DNA extraction to dots of dye

4.    Load samples into wells (note the gel needs to be in buffer at the time)

5.    Run samples at 140V until dye runs ~2cm from wells

6.    Take tray out of rig and take picture with UV camera

7.    Dilute samples with H₂O if necessary according to sample brightness